BD Pharmingen- Biotin Rat Anti-Mouse IL-4_BD Pharmingen
产品信息
抗原名称
IL-4
宿主
Rat IgG1
免疫原
Recombinant IL-4
简单描述
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD6-24G2 hybridoma was recombinant IL-4. This is a non-neutralizing antibody.
This antibody is routinely tested by ELISA Detection. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
BVD6-24G2
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD6-24G2 hybridoma was recombinant IL-4. This is a non-neutralizing antibody.
This antibody is routinely tested by ELISA Detection. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG1
克隆号
克隆 BVD6-24G2 (RUO)
浓度
0.5 mg/ml
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
ELISA Detection (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IL-4
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA).
2. Bogen SA, Fogelman I, Abbas AK. Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens. J Immunol. 1993; 150(10):4197-4205. (Clone-specific: ELISA).
3. Chatelain R, Varkila K, Coffman RL. IL-4 induces a Th2 response in Leishmania major-infected mice. J Immunol. 1992; 148(4):1182-1187. (Clone-specific: ELISA).
4. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA).
5. Shirai A, Sierra V, Kelly CI, Klinman DM. Individual cells simultaneously produce both IL-4 and IL-6 in vivo. Cytokine. 1994; 6(3):329-336. (Clone-specific: ELISA).
数据库链接
Entrez-Gene ID
16189
参考图片
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