产品信息
荧光素标记
PE
抗原名称
IL-4
宿主
Rat IgG2b
免疫原
Recombinant Mouse IL-4
简单描述
IL-4 is a pleiotropic cytokine that can regulate immunological responses including cell proliferation, survival and gene expression. Expressed by mast cells, basophils, eosinophils and T-cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells. IL-4 can also be involved with the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes in addition to being involved with the development of allergic inflammation and asthma.
商品描述
IL-4 is a pleiotropic cytokine that can regulate immunological responses including cell proliferation, survival and gene expression. Expressed by mast cells, basophils, eosinophils and T-cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells. IL-4 can also be involved with the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes in addition to being involved with the development of allergic inflammation and asthma.
同种型
Rat IgG2b
克隆号
BVD4-1D11
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Mouse (QC Testing)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization).
2. Finkelman FD, Madden KB, Morris SC, et al. Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes. J Immunol. 1993; 151(3):1235-1244. (Clone-specific: ELISA, Neutralization).
3. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Clone-specific: ELISA).
4. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology).
5. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA, Neutralization).
参考图片
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