BD Pharmingen- Purified NA-LE Hamster Anti-Mouse MCP-1_BD Pharmingen
产品信息
抗原名称
MCP-1
宿主
Armenian Hamster IgG1, κ
免疫原
Heparin-purified CHO-expressed mouse MCP-1
简单描述
The 2H5 antibody reacts with mouse and rat monocyte chemoattractant protein (MCP-1), formerly termed JE. This antibody also recognizes human MCP-1, but shows no reactivity with the closely related mouse β chemokines, TCA3 and MIP-1β. The immunogen used to generate the 2H5 hybridoma was heparin-purified CHO-expressed mouse MCP-1. This is a neutralizing antibody.
商品描述
2H5
The 2H5 antibody reacts with mouse and rat monocyte chemoattractant protein (MCP-1), formerly termed JE. This antibody also recognizes human MCP-1, but shows no reactivity with the closely related mouse β chemokines, TCA3 and MIP-1β. The immunogen used to generate the 2H5 hybridoma was heparin-purified CHO-expressed mouse MCP-1. This is a neutralizing antibody.
同种型
Armenian Hamster IgG1, κ
克隆号
克隆 2H5 (RUO)
浓度
1.0 mg/ml
产品详情
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
应用
实验应用
ELISA (Routinely Tested), Intracellular staining (flow cytometry), Neutralization (Tested During Development)
反应种属
Mouse (QC Testing)
目标/特异性
MCP-1
制备和贮存
存储溶液
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
保存方式
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
文献
文献
研发参考(3)
1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Clone-specific).
2. Luo Y, Laning J, Hayashi M, Hancock PR, Rollins B, Dorf ME. Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1. J Immunol. 1994; 153(8):3708-3716. (Clone-specific).
3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Apoptosis).
参考图片
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