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BD Pharmingen- PE Mouse Anti-Human IFN_BD Pharmingen

艾美捷科技 • 2025年08月22日 11:15:13 • 抗体 • 阅读 71 • 标签
产品信息
荧光素标记
PE
抗原名称
IFN-γ
宿主
Mouse BALB/c IgG1, κ
免疫原
Human IFN-γ from supernatants of S. aureus-stimulated PBMC
简单描述
The 4S.B3 monoclonal antibody specifically binds to interferon-γ (IFN-γ). The immunogen used to generate this hybridoma was partially purified human IFN-γ obtained from supernatants of human PBMC stimulated with Staphylococcus aureus . Interferon-γ (IFN-γ) is a potent multifunctional cytokine that is produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ Receptor Complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γ is a 14-18 kDa glycoprotein containing 143 amino acid residues. Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ + cells within a mixed cell population.
商品描述
4S.B3 The 4S.B3 monoclonal antibody specifically binds to interferon-γ (IFN-γ). The immunogen used to generate this hybridoma was partially purified human IFN-γ obtained from supernatants of human PBMC stimulated with Staphylococcus aureus . Interferon-γ (IFN-γ) is a potent multifunctional cytokine that is produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ Receptor Complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γ is a 14-18 kDa glycoprotein containing 143 amino acid residues. Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ + cells within a mixed cell population.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 4S.B3 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
IFN-γ
背景
别名
IFNG; Interferon-gamma; IFG; IFI; Type II interferon
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
3458

参考图片

Expression of IFN-γ by stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 6 hr with PMA (50 ng/ml; Sigma) and calcium ionophore A23187 (500 ng/ml; Sigma) in the presence of 2 µM BD GolgiStop™ (Cat. No. 554724). The PBMC were stained with PE-Cy™5 Mouse Anti-Human CD3 (Cat. No. 555334/561007), fixed, permeabilized, and subsequently stained with 0.125 µg of PE Mouse Anti-Human IFN-γ (Cat. No. 554552/557074/559326/561056). The binding of PE Mouse Anti-Human IFN-γ was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-γ (Cat. No. 554549, 5 µg; right panel). The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls.

Expression of IFN-γ by stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 6 hr with PMA (50 ng/ml; Sigma) and calcium ionophore A23187 (500 ng/ml; Sigma) in the presence of 2 µM BD GolgiStop™ (Cat. No. 554724). The PBMC were stained with PE-Cy™5 Mouse Anti-Human CD3 (Cat. No. 555334/561007), fixed, permeabilized, and subsequently stained with 0.125 µg of PE Mouse Anti-Human IFN-γ (Cat. No. 554552/557074/559326/561056). The binding of PE Mouse Anti-Human IFN-γ was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-γ (Cat. No. 554549, 5 µg; right panel). The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls.

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