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BD Pharmingen- PE Mouse Anti-Human IL-1_BD Pharmingen

艾美捷科技 • 2025年08月22日 11:18:38 • 抗体 • 阅读 12 • 标签
产品信息
荧光素标记
PE
抗原名称
IL-1α
宿主
Mouse IgG1, κ
免疫原
recombinant human IL-1α
简单描述
The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The immunogen used to generate the 364-3B3-14 hybridoma was recombinant human IL-1α. The 364-3B3-14 antibody does not cross-react with human IL-1β. This is a non-neutralizing antibody.
商品描述
364-3B3-14 The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The immunogen used to generate the 364-3B3-14 hybridoma was recombinant human IL-1α. The 364-3B3-14 antibody does not cross-react with human IL-1β. This is a non-neutralizing antibody.
同种型
Mouse IgG1, κ
克隆号
克隆 364-3B3-14 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
IL-1α
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). 2. Thorpe R, Wadhwa M, Gearing A, Mahon B, Poole S. Sensitive and specific immunoradiometric assays for human interleukin-1 alpha. Lymphokine Res. 1988; 7(2):119-127. (Clone-specific).

参考图片

Expression of IL-α by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS (10 ng/ml final concentration) in the presence of GolgiStop™ (2 µM final concentration; Cat. No. 554724). The PBMC were stained with FITC-mouse anti-human CD14 antibody (Clone M5E2, Cat. No. 555397) fixed/permeabilized, and then stained with 0.25 µg of PE-mouse anti-human IL-1α antibody (PE-364-3B3-14, Cat. No. 554561), (left panel). The data reflects gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE-364-3B3-14 was blocked by preincubation of the PE-364-3B3-14 with a molar excess of recombinant human IL-1α (middle panel), and by the preincubation of the fixed/permeabilized cells with an excess of the unlabeled \"cold\" 364-3B3-14 mouse antibody (5 µg; Cat. No. 551223; right panel). The quadrant markers for the bivariate dot plots were set based on the fixed/permeabilized unstained cell controls, and verified with the recombinant cytokine and \"cold\" antibody specificity controls.

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