Interleukin-3 (IL-3) is a species-specific cytokine which acts on hematopoietic progenitors of every lineage, with the probable exception of cells committed to the lymphoid lineage.  It has been referred to as CSF-2 , BPA, megakaryocyte CSF, HCGF and hemopoietin 2.  Mouse IL-3 is a 17.2 kD protein containing 140 amino acid residues.    Recombinant mouse IL-3 (Cat. No. 554579) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives.  Recombinant mouse IL-3 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of mouse IL-3, as measured in a chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Mouse (QC Testing)

IL-3

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(6) 1. Frendl G. Interleukin 3: from colony-stimulating factor to pluripotent immunoregulatory cytokine. Int J Immunopharmacol. 1992; 14:421-430. (Biology: IC/FCM Block). 2. Fung MC, Hapel AJ, Ymer S, et al. Molecular cloning of cDNA for murine interleukin-3. Nature. 1984; 307(5948):233-237. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 4. Urdal DL, Mochizuki D, Conlon PJ, et al. Lymphokine purification by reversed-phase high-performance liquid chromatography. J Chromatogr. 1984; 296:171-179. (Biology). 5. Weinstein Y, Ihle JN, Lavu S, Reddy EP. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc Natl Acad Sci U S A. 1986; 83(14):5010-5014. (Biology). 6. Yokota T, Lee F, Rennick D, et al. Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc Natl Acad Sci U S A. 1984; 81(4):1070-1074. (Biology).