Interleukin-5 (IL-5) is a lymphokine responsible for the activities attributed to eosinophil differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF).  IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation.  Mouse IL-5 presents as a 35-37 kD homodimer (in non-reduced gel).  IL-5 promotes the growth and differentiation of hematopoietic cells, primarily of the eosinophil lineage.  IL-5 stimulates the generation of cytotoxic T cells and supports the growth and differentiation of pre-activated B cells.  Recombinant mouse IL-5 (Cat. No. 554581) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives.  Recombinant mouse IL-5 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng/µg of mouse IL-5, as measured in a chromogenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Mouse (QC Testing)

IL-5

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(4) 1. Kinashi T, Harada N, Severinson E, et al. Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II. Nature. 1986; 324(6092):70-72. (Biology). 2. Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Methodology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 4. Sideras P, Noma T, Honjo T. Structure and function of interleukin 4 and 5. Immunol Rev. 1988; 102:189-212. (Biology).