Mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine made by activated T cells, macrophages, vascular endothelial cells and fibroblasts.  It has potent stimulatory effects on the growth and differentiation of bone marrow progenitor cells that generate granulocytes, monocytes/macrophages, and megakaryocytes.  In peripheral tissues, GM-CSF can act on mature leukocytes to promote inflammatory responses.  Mouse GM-CSF runs as a 15.5 - 19 kD band in reducing and non-reducing SDS-PAGE analysis.  Mouse GM-CSF protein contains 124 amino acid residues.  Recombinat mouse GM-CSF (Cat. No. 554586) is supplied as a frozen liquid comprised of 0.22 um sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives.  Recombinant mouse GM-CSF is ≥ 95% pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng per µg of mouse GM-CSF, as measured in chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Mouse (QC Testing)

GM-CSF

Frozen aqueous buffered solution containing BSA.

Development References(6) 1. Gasson JC. Molecular physiology of granulocyte-macrophage colony-stimulating factor. Blood. 1991; 77(6):1131-1145. (Biology). 2. Gough NM, Gough J, Metcalf D, et al. Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor. Nature. 1984; 309(5971):763-767. (Biology). 3. Miyatake S, Otsuka T, Yokota T, Lee F, Arai K. Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes. EMBO J. 1985; 4(10):2561-2568. (Biology). 4. Price V, Mochizuki D, March CJ, et al. Expression, purification and characterization of recombinant murine granulocyte-macrophage colony-stimulating factor and bovine interleukin-2 from yeast. Gene. 1987; 55:287-293. (Biology). 5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). 6. Thompson-Snipes L, Dhar V, Bond MW, Mosmann TR, Moore KW, Rennick DM. Interleukin 10: a novel stimulatory factor for mast cells and their progenitors. J Exp Med. 1991; 173(2):507-510. (Methodology).