Interleukin-6 (IL-6), also known as IFN-β2, BSF-2, and HPGF, is a multifunctional cytokine which regulates immune responses, acute-phase reactions and hematopoiesis.  Reported cellular targets for IL-6 action include hepatocytes, B and T lymphocytes, neurons, tumor cells and multipotent hematopoietic cells.  Mouse IL-6 is a 21.7 kD protein containing 187 amino acid residues.  Recombinant mouse IL-6 (Cat. No. 554582) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives.  Recombinant mouse IL-6 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng per µg of mouse IL-6, as measured in a chromogenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Mouse (QC Testing)

IL-6

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(5) 1. Chiu CP, Moulds C, Coffman RL, Rennick D, Lee F. Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells. Proc Natl Acad Sci U S A. 1988; 85(19):7099-7103. (Biology). 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 3. Van Snick J, Cayphas S, Szikora JP, et al. cDNA cloning of murine interleukin-HP1: homology with human interleukin 6. Eur J Immunol. 1988; 18(2):193-197. (Biology). 4. Van Snick J, Cayphas S, Vink A, et al. Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. Proc Natl Acad Sci U S A. 1986; 83(24):9679-9683. (Biology). 5. Weinstein Y, Ihle JN, Lavu S, Reddy EP. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc Natl Acad Sci U S A. 1986; 83(14):5010-5014. (Methodology).