Tumor Necrosis Factor (TNF, aka TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells.  Mouse TNF is a differentially glycosylated protein containing 156 amino acid residues.  Recombinant mouse TNF (Cat. No. 554589) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives.  Recombinant mouse TNF is ≥ 95% pure as determined by SDS-PAGE, and an absorption assay based on the Beers-Lambert Law.  The endotoxin level is ≤ 0.1 ng per µg of mouse TNF, as measured in a chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Mouse (QC Testing)

TNF

Frozen aqueous buffered solution containing BSA.

Development References(3) 1. Hogan MM, Vogel SN. Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins. J Immunol. 1988; 141(12):4196-4202. (Methodology: Bioassay). 2. Jaattela, M. . Biologic activities and mechanisms of action of tumor necrosis factor-α/cachectin. Lab Invest. 1991; 64:724-742. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology).