Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a member of the CC chemokine family and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells. MCP-1 has chemoattractant activity on monocytes, lymphocytes and basophils but is not active on neutrophils.  Recombinant mouse MCP-1 (MN 554590) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 1.29 mg/mL bovine serum albumin, with no preservatives.  Recombinant mouse MCP-1 is > 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of mouse MCP-1, as measured in a chromogenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Mouse (QC Testing)

MCP-1

Frozen aqueous buffered solution containing BSA.

Development References(7) 1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Methodology). 2. Cochran BH, Reffel AC, Stiles CD. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 1983; 33(3):939-947. (Biology). 3. Ernst CA, Zhang YJ, Hancock PR, Rutledge BJ, Corless CL, Rollins BJ. Biochemical and biologic characterization of murine monocyte chemoattractant protein-1. Identification of two functional domains.. J Immunol. 1994; 152(7):3541-3549. (Biology). 4. Luo Y, Laning J, Hayashi M, Hancock PR, Rollins B, Dorf ME. Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1. J Immunol. 1994; 153(8):3708-3716. (Biology). 5. Rollins BJ, Morrison ED, Stiles CD. Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties. Proc Natl Acad Sci U S A. 1988; 85(11):3738-3742. (Biology). 6. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). 7. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p.