Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses.  Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits.  IL-12 binds to specific high affinity receptors and can; i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells; ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells; iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and; iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.  Recombinant mouse IL-12 p70 (Cat. No. 554592) is provided lyophilized, containing bovine serum albumin and no preservatives.  Recombinant mouse IL-12 p70 was found to be ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beer-Lambert law.  The endotoxin level is ≤ 0.1 ng per μg of mouse IL-12 p70, as measured in a chromogenic LAL assay.

(RUO)

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Mouse (QC Testing)

IL-12

Lyophilized in an aqueous buffered solution containing BSA.

Development References(3) 1. Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology). 2. Schoenhaut DS, Chua AO, Wolitzky AG, et al. Cloning and expression of murine IL-12. J Immunol. 1992; 148(11):3433-3440. (Biology). 3. Zeh HJ III, Tahara H, Lotze MT. Interleukin-12. In: Thomson AW, ed. The Cytokine Handbook. London: Academic Press Limited; 1994:239-256.