Interleukin-1β (IL-1β) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. This is the mature form of human IL-1β, a 17 kD protein containing 153 amino acid residues, representing amino acids A117 through S269 of the IL-1β precursor protein, expressed in E. coli .  Recombinant human IL-1β (Cat. No. 554602) is lyophilized from a solution comprised of 0.22 μm sterile-filtered aqueous buffered solution with no preservatives.  Recombinant human IL-1β is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 1.0 EU per µg of human IL-1β as measured in a chromogenic LAL assay.

(RUO)

ELISA (Routinely Tested), Bioassay (Tested During Development)

Human (QC Testing)

IL-1β

Lyophilized in an aqueous buffered solution containing BSA.

Development References(4) 1. Auron PE, Webb AC, Rosenwasser LJ, et al. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc Natl Acad Sci U S A. 1984; 81(24):7907-7911. (Biology). 2. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). 3. Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Methodology). 4. Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Methodology).