Interleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multifunctional cytokine which can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages.  IL-2 exerts its biological effects by binding to specific receptors expressed by various target cells.  Human IL-2 is a 15 kD protein containing 133 amino acid residues.  Recombinant human IL-2 (Cat. No. 554603) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing glycerol and bovine serum albumin, with no preservatives.  Recombinant human IL-2 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng per µg of human IL-2, as measured in a chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Intracellular block/flow cytometry (Tested During Development)

Human (QC Testing)

IL-2

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(5) 1. Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Biology). 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 3. Smith, KA. Interleukin-2: inception, impact, and implications. Science. 1988; 240(4856):1169-1176. (Biology). 4. Stern AS, Pan YC, Urdal DL, et al. Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia. Proc Natl Acad Sci U S A. 1984; 81(3):871-875. (Biology). 5. Taniguchi T, Matsui H, Fujita T, et al. Structure and expression of a cloned cDNA for human interleukin-2. Nature. 1983; 302(5906):305-310. (Biology).