Interleukin-8 (IL-8) is an 8 kD protein containing 72 amino acid residues. It promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. Formulation and Purity Purified human IL-8 was found to be > 98% pure as measured by SDS-PAGE analysis and an absorbance assay based on the Beers-Lambert law. It possesses ≤ 0.1 ng endotoxin per µg human IL-8 as measured using a chromogenic LAL assay. Recombinant human IL-8 is a lyophilized aqueous buffered solution containing 50 µg bovine serum albumin per 1µg recombinant human IL-8. The product contains no preservatives.

(RUO)

ELISA Standard (Routinely Tested), Functional assay (Tested During Development)

IL-8

Lyophilized in an aqueous buffered solution containing BSA.

Development References(3) 1. Bauser R, Proudfoot AE. Calcium Mobilization. In: Proudfoot AE, Wells TN, Power CA, ed. Chemokine Protocols, Volume 138. Totowa, New Jersey: Humana Press; 2000:143-148. 2. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Blocking).