Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI.  The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.  The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.  Recombinant human IL-10 (Cat No. 554611) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, containing glycerol and bovine serum albumin, with no preservatives.  Recombinant human IL-10 is ≥ 95% pure as determined by SDS-PAGE  analysis, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng per µg of human IL-10, as measured in a chromogenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Human (QC Testing)

IL-10

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(3) 1. Howard M, O'Garra A, Ishida H, de Waal Malefyt R, de Vries J. Biological properties of interleukin 10. J Clin Immunol. 1992; 12(4):239-247. (Biology). 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 3. Vieira P, de Waal-Malefyt R, Dang MN, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. Proc Natl Acad Sci U S A. 1991; 88(4):1172-1176. (Biology).