Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells.  IFN-γ exerts its biological effects through specific binding to a single class of high affinity receptors.  In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils.  In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets.  These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.  Human IFN-γ is a 14 - 18 kD multiple glycosylated form protein containing 143 amino acid residues.  Recombinant human IFN-γ (Cat. No. 554616) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives.  Human IFN-γ is ≥ 95% pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng/μg of human IFN-γ, as measured in a chromogenic LAL assay.

(RUO)

250 µg/ml

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Human (QC Testing)

IFN-γ

Frozen aqueous buffered solution containing BSA.

Development References(4) 1. Farrar MA, Schreiber RD. The molecular cell biology of interferon-gamma and its receptor. Annu Rev Immunol. 1993; 11:571-611. (Biology). 2. Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 4. Vogel S, Friedman R, Hogan M. Measurement of antiviral activity induced by interferons a, b, and g.. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley & Sons; 2007:6.9.1-6.9.15.