Tumor Necrosis Factor (TNF, also known as TNF-α) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF is a 17.5 kD protein containing 157 amino acid residues.  Recombinant human TNF (Cat. No. 554618) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives.  Recombinant human TNF is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng/μg of human TNF, as measured in a chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Human (QC Testing)

TNF

Frozen aqueous buffered solution containing BSA.

Development References(4) 1. Hogan MM, Vogel SN. Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins. J Immunol. 1988; 141(12):4196-4202. (Biology). 2. Jaattela, M. . Biologic activities and mechanisms of action of tumor necrosis factor-α/cachectin. Lab Invest. 1991; 64:724-742. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 4. Wang AM, Creasey AA, Ladner MB, et al. Molecular cloning of the complementary DNA for human tumor necrosis factor. Science. 1985; 228(4696):149-154. (Biology).