Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.  Recombinant human MCP-1 (Cat. No. 554620) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives.  Recombinant human MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is ≤ 0.1 ng/µg of human MCP-1, as measured in a chromogenic LAL assay.

(RUO)

200 µg/ml

ELISA (Routinely Tested), Bioassay (Tested During Development)

MCP-1

Frozen aqueous buffered solution containing BSA.

Development References(5) 1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Biology). 2. Rollins BJ, Stier P, Ernst T, Wong GG. The human homolog of the JE gene encodes a monocyte secretory protein. Mol Cell Biol. 1989; 9(11):4687-4695. (Biology). 3. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). 4. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p. 5. Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 1989; 244(2):487-493. (Biology).