This suspension contains Mouse intracellular CytoKine-3   (MiCK-3) Positive Control Cells. The MiCK-3 cell suspension contains fixed, non-permeabilized mouse lymphoid cells. The suspension includes cells that express easily detectable levels of intracellular IL-1α, IL-6, IL-12, MCP-1 or TNF  as determined by immunofluorescent staining of intracellular cytokines and flow cytometry. MiCK-3 cell suspensions were prepared by stimulating mouse macrophages in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and were incubated with Fc Block™ [rat IgG2b,κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide (DMSO) and 90% fetal bovine serum at -80°C. Each vial of MiCK-3 contains measurable proportions of cytokine-producing cells.  Representative flow cytometric results are shown for typical staining of MiCK-3 Positive Control Cells. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.

This suspension contains Mouse intracellular CytoKine-3   (MiCK-3) Positive Control Cells. The MiCK-3 cell suspension contains fixed, non-permeabilized mouse lymphoid cells. The suspension includes cells that express easily detectable levels of intracellular IL-1α, IL-6, IL-12, MCP-1 or TNF  as determined by immunofluorescent staining of intracellular cytokines and flow cytometry. MiCK-3 cell suspensions were prepared by stimulating mouse macrophages in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and were incubated with Fc Block™ [rat IgG2b,κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide (DMSO) and 90% fetal bovine serum at -80°C. Each vial of MiCK-3 contains measurable proportions of cytokine-producing cells.  Representative flow cytometric results are shown for typical staining of MiCK-3 Positive Control Cells. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.

Mouse IgG1, κ

Intracellular staining (flow cytometry) (Routinely Tested)

Frozen in FBS and 10% DMSO.

研发参考(2) 1. BD Biosciences. Techniques for Immune Function Analysis, Application Handbook 1st Edition. 2003. Available: http://www.bdbiosciences.com/pdfs/manuals/02-8100055-21A1rr.pdf 2007, Jan. 25. 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block).