1. 首页
  2. 抗体
  3. 正文

BD Pharmingen- MiCK-2 Mouse Cytokine Positive Control Cells_BD Pharmingen

艾美捷科技 • 2025年08月22日 13:06:06 • 抗体 • 阅读 57 • 标签
产品信息
简单描述
This suspension contains Mouse intracellular CytoKine-2 (MiCK-2) Positive Control Cells. The MiCK-2 cell suspension contains fixed, non-permeabilized mouse lymphoid cells (20 test samples at 2.5 x 10^5 cells/test) in 1 mL final volume. The suspension includes cells that express easily detectable levels of intracellular IL-4, IL-10 or GM-CSF as determined by immunofluorescent staining of intracellular cytokines and flow cytometry. MiCK-2 cell suspensions were prepared by stimulating mouse spleen cells in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and were incubated with BD FcBlock™ [rat IgG2b, κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide (DMSO) and 90% fetal bovine serum at -80°C. Each vial of MiCK-2 contains measurable proportions of cytokine- producing cells.  Representative flow cytometric results are shown for typical staining of MiCK-2 Positive Control Cells. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.
商品描述
This suspension contains Mouse intracellular CytoKine-2 (MiCK-2) Positive Control Cells. The MiCK-2 cell suspension contains fixed, non-permeabilized mouse lymphoid cells (20 test samples at 2.5 x 10^5 cells/test) in 1 mL final volume. The suspension includes cells that express easily detectable levels of intracellular IL-4, IL-10 or GM-CSF as determined by immunofluorescent staining of intracellular cytokines and flow cytometry. MiCK-2 cell suspensions were prepared by stimulating mouse spleen cells in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and were incubated with BD FcBlock™ [rat IgG2b, κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide (DMSO) and 90% fetal bovine serum at -80°C. Each vial of MiCK-2 contains measurable proportions of cytokine- producing cells.  Representative flow cytometric results are shown for typical staining of MiCK-2 Positive Control Cells. Investigators should anticipate similar (though not identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.
克隆号
(RUO)
浓度
5x10^6 cells/ml
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
目标/特异性
Mick-2 Cytokine positive control cells
制备和贮存
存储溶液
Frozen in FBS and 10% DMSO.
保存方式
Frozen in FBS and 10% DMSO.
文献
文献
研发参考(2) 1. BD Biosciences. Techniques for Immune Function Analysis, Application Handbook 1st Edition. 2003. Available: http://www.bdbiosciences.com/pdfs/manuals/02-8100055-21A1rr.pdf 2007, Jan. 25. 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block).

参考图片

Characteristic staining of MiCK-2 Positive Control Cells with anti-mouse IL-4, IL-10 and GM-CSF. MiCK-2 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control (PE-R3-34; Cat. No. 554685; Panel A), PE-rat anti-mouse IL-4 (PE-11B11, Cat. No. 554435; Panel B), PE-rat anti-mouse IL-10 (PE-JES5-16E3, Cat. No. 554467; Panel C) or PE-rat anti-mouse GM-CSF (PE-MP1-22E9, Cat. No. 554406; Panel D). Despite fixation and freezing, the side-and forward-scattered light signals for these control cells (Panel F) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown in Panels A-D).

Characteristic staining of MiCK-2 Positive Control Cells with anti-mouse IL-4, IL-10 and GM-CSF.   MiCK-2 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control (PE-R3-34; Cat. No. 554685; Panel A), PE-rat anti-mouse IL-4 (PE-11B11, Cat. No. 554435; Panel B), PE-rat anti-mouse IL-10 (PE-JES5-16E3, Cat. No. 554467; Panel C) or PE-rat anti-mouse GM-CSF (PE-MP1-22E9, Cat. No. 554406; Panel D). Despite fixation and freezing, the side-and forward-scattered light signals for these control cells (Panel F) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown in Panels A-D).

文档下载: W 导出为BD Pharmingen- MiCK-2 Mouse Cytokine Positive Control Cells_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9696.html

赞 (57)
打赏 微信扫一扫
« 上一篇 2025年08月22日 13:05:27
下一篇 » 2025年08月22日 13:06:28

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();