BD Pharmingen- Purified NA-LE Mouse Anti-Human IL-8_BD Pharmingen
产品信息
抗原名称
IL-8
宿主
Mouse IgG1, κ
免疫原
Recombinant human IL-8
简单描述
The 2A2 antibody reacts with both the 72 and 77 amino acid forms of human IL-8. The immunogen used to produce the 2A2 hybridoma was E. coli-expressed recombinant human IL-8 (77 amino acid form).
This antibody has been reported to show no cross-reactivity with the following cytokines or chemokines: Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-12 (p40 or p70), IL-13, IL-15, eotaxin, GRO, IFN- γ, MCP-1, MCP-2, MCP-3, MIP-1α, MIP-1β, NAP-2, PF4, RANTES, SCF, TNF, LT-α.
Neutralization:
Investigators are advised that this material is not routinely tested for the Neutralization application and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. This antibody has previously been reported to be useful for the neutralization of recombinant human IL-8 when measured with a calcium flux assay using 250 ng/mL recombinant human IL-8 (Cat. No. 554609) to stimulate calcium mobilization in 1x10^7 cells/mL human lysed whole blood as indicator cells. Preincubation of the antibody with recombinant human IL-8 can neutralize calcium mobilization with the following representative ranges:
50% Neutralization (ND50) at 8 - 10 μg/mL
> 95% Neutralization at 150 - 500 μg/mL
商品描述
2A2
The 2A2 antibody reacts with both the 72 and 77 amino acid forms of human IL-8. The immunogen used to produce the 2A2 hybridoma was E. coli-expressed recombinant human IL-8 (77 amino acid form).
This antibody has been reported to show no cross-reactivity with the following cytokines or chemokines: Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-12 (p40 or p70), IL-13, IL-15, eotaxin, GRO, IFN- γ, MCP-1, MCP-2, MCP-3, MIP-1α, MIP-1β, NAP-2, PF4, RANTES, SCF, TNF, LT-α.
Neutralization:
Investigators are advised that this material is not routinely tested for the Neutralization application and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. This antibody has previously been reported to be useful for the neutralization of recombinant human IL-8 when measured with a calcium flux assay using 250 ng/mL recombinant human IL-8 (Cat. No. 554609) to stimulate calcium mobilization in 1x10^7 cells/mL human lysed whole blood as indicator cells. Preincubation of the antibody with recombinant human IL-8 can neutralize calcium mobilization with the following representative ranges:
50% Neutralization (ND50) at 8 - 10 μg/mL
> 95% Neutralization at 150 - 500 μg/mL
同种型
Mouse IgG1, κ
克隆号
克隆 2A2 (RUO)
浓度
1.0 mg/ml
产品详情
NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
应用
实验应用
ELISA (Routinely Tested), Neutralization (Tested During Development), Western blot (Reported)
反应种属
Human (QC Testing)
目标/特异性
IL-8
制备和贮存
存储溶液
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
保存方式
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
文献
文献
研发参考(2)
1. Leonard EJ, Sylvester I, Yoshimura T, et al. Current Protocols in Immunology. John Wiley and Sons; 1995:6.12.1-6.12.26.
2. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Clone-specific).
参考图片
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