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BD Pharmingen- PE Mouse Anti-Human MIP-1- -CCL3_BD Pharmingen

艾美捷科技 • 2025年08月22日 13:49:05 • 抗体 • 阅读 86 • 标签
产品信息
荧光素标记
PE
抗原名称
MIP-1α
宿主
Mouse IgG2a, κ
简单描述
The 11A3 monoclonal antibody specifically recognizes macrophage inflammatory protein-1α (MIP-1α), a member of the CC chemokine family of proteins. It is produced by T cells, B cells, Langerhans cells, neutrophils and macrophages. MIP-1α plays a role as an inhibitor of stem cell proliferation and as a chempattractant of B cells, eosinophils and cytotoxic T cells. Clone 11A3 also cross-reacts with an intracellular component of rhesus and cynomolgus macaque-LPS-stimulated peripheral blood monocytes. The reactivity pattern observed on CD14-positive cells is similar to that seen on normal human peripheral blood monocytes.
商品描述
11A3 The 11A3 monoclonal antibody specifically recognizes macrophage inflammatory protein-1α (MIP-1α), a member of the CC chemokine family of proteins. It is produced by T cells, B cells, Langerhans cells, neutrophils and macrophages. MIP-1α plays a role as an inhibitor of stem cell proliferation and as a chempattractant of B cells, eosinophils and cytotoxic T cells. Clone 11A3 also cross-reacts with an intracellular component of rhesus and cynomolgus macaque-LPS-stimulated peripheral blood monocytes. The reactivity pattern observed on CD14-positive cells is similar to that seen on normal human peripheral blood monocytes.
同种型
Mouse IgG2a, κ
克隆号
克隆 11A3 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence (Tested During Development)
反应种属
Human (QC Testing), Rhesus, Cynomolgus (Tested in Development)
目标/特异性
MIP-1α
背景
别名
CCL3
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4) 1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology: Flow cytometry). 2. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology). 3. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p. 4. Wolpe SD, Cerami A. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J. 1989; 3(14):2565-2573. (Biology).

参考图片

Flow cytometric analysis of MIP-1α (CCL3) expression by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hr with LPS (10 ng/ml) in the presence of GolgiStop™ (Cat. No. 554724; aka monensin 2 µM). The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 (Cat. No. 555397), fixed, permeabilized, and subsequently stained with 0.25 µg of PE Mouse Anti-Human MIP-1α (CCL3) (Cat. No. 554730) following Pharmingen's staining protocol (left panel). The data reflect gating on monocytes, based on forward and side light scatter. To demonstrate specificity of staining, the binding of PE Mouse Anti-Human MIP-1α (CCL3) was blocked by the preincubation of the conjugated antibody with recombinant human MIP-1α (0.5 µg; middle panel), and by preincubation of the fixed/permeabilized cells with the unlabeled 11A3 antibody (5.0 µg, right panel) prior to staining with the conjugate antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody (right panel) blocking specificity controls.

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