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BD Pharmingen- FITC Mouse Anti-Rat Granulocytes_BD Pharmingen

艾美捷科技 • 2025年08月22日 14:25:07 • 抗体 • 阅读 15 • 标签
产品信息
荧光素标记
FITC
抗原名称
Granulocyte
宿主
Mouse IgM, κ
免疫原
PVG Rat Spleen Cells
简单描述
The HIS48 antibody reacts with an antigen that is variably expressed on rat granulocytes and monocyte subsets. The HIS48 antibody also recognizes developing cells of the erythroid cell lineage. PVG rat spleen cells were used as the immunogen to generate the hybridoma that produces the HIS48 antibody.
商品描述
HIS48 The HIS48 antibody reacts with an antigen that is variably expressed on rat granulocytes and monocyte subsets. The HIS48 antibody also recognizes developing cells of the erythroid cell lineage. PVG rat spleen cells were used as the immunogen to generate the hybridoma that produces the HIS48 antibody.
同种型
Mouse IgM, κ
克隆号
克隆 HIS48 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Rat (QC Testing)
目标/特异性
Granulocytes and Erythroid Cells
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(1) 1. van Goor H, Fidler V, Weening JJ, Grond J. Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids. Lab Invest. 1991; 64(6):754-765. (Clone-specific).

参考图片

Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody (Rat BD Fc Block™) [Cat. No. 550270/550271). The cells were then stained with either FITC Mouse IgM, κ Isotype Control (Cat. No. 553474; Left Plot) or FITC Mouse Anti-Rat Granulocytes antibody (Cat. No. 554907; Right Plot) at 0.125 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto™ II Flow Cytometry System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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