BD Pharmingen- Purified Mouse Anti-Rat - T-Cell Receptor_BD Pharmingen
产品信息
抗原名称
TCR αβ
宿主
Mouse BALB/c IgG1, κ
免疫原
Rat T blasts and rat erythrocytes
简单描述
The R73 monoclonal antibody specifically recognizes the αβ T-cell Receptor (TCR) found on most peripheral T lymphocytes, intestinal intraepithelial lymphocytes, and thymocytes. It does not react with γδ TCR-bearing cells. Cross-linked R73 mAb induces T-cell activation and differentiation. In vivo treatment with mAb R73 can suppress immune function of peripheral αβ TCR-expressing T cells, and reduce the severity of experimental autoimmune, transplant rejection, and graft-versus-host responses.
商品描述
R73
The R73 monoclonal antibody specifically recognizes the αβ T-cell Receptor (TCR) found on most peripheral T lymphocytes, intestinal intraepithelial lymphocytes, and thymocytes. It does not react with γδ TCR-bearing cells. Cross-linked R73 mAb induces T-cell activation and differentiation. In vivo treatment with mAb R73 can suppress immune function of peripheral αβ TCR-expressing T cells, and reduce the severity of experimental autoimmune, transplant rejection, and graft-versus-host responses.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 R73 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), (Co)-stimulation, Cytotoxicity, Depletion, Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed, Immunoprecipitation, Stimulation, Western blot (Reported)
反应种属
Rat (QC Testing)
目标/特异性
TCR αβ
背景
别名
TCR α/β: TCR αβ; αβ TCR; alpha beta TCR
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(14)
1. Brenan M, Rees DJ. Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 1997; 27(11):3070-3079. (Clone-specific: Western blot).
2. Fangmann J, Schwinzer R, Wonigeit K. Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor alpha/beta+/CD2- cells and high expression of the RT6 alloantigen. Eur J Immunol. 1991; 21(3):753-760. (Biology).
3. Gaede K, Nazet M, Bosse D, Hunig, Heesemann J. Treatment of arthritis in Lewis rats by a monoclonal antibody against alpha beta T cell receptor: differential sensitivity of Yersinia-induced arthritis versus adjuvant arthritis. Clin Immunol Immunopathol. 1995; 77(3):339-348. (Clone-specific: Depletion, Immunohistochemistry).
4. Hasegawa T, Tanaka T, Yoshikai Y. The appearance and role of gamma delta T cells in the peritoneal cavity and liver during primary infection with Listeria monocytogenes in rats. Int Immunol. 1992; 4(10):1129-1136. (Clone-specific: Depletion).
5. Heidecke CD, Hancock WW, Jakobs F, et al. alpha/beta-T cell receptor-directed therapy in rat cardiac allograft recipients. Treatment prior to alloantigen exposure prevents sensitization and abrogates accelerated rejection. Transplantation. 1995; 59(1):78-84. (Clone-specific: Depletion, Immunohistochemistry).
6. Heidecke CD, Hancock WW, Westerholt S, et al. alpha/beta-T cell receptor-directed therapy in rat allograft recipients. Long-term survival of cardiac allografts after pretreatment with R73 mAb is associated with upregulation of Th2-type cytokines. Transplantation. 1996; 61(6):948-956. (Clone-specific: Depletion, Immunohistochemistry).
7. Hunig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G. A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989; 169(1):73-86. (Immunogen: (Co)-stimulation, Immunohistochemistry, Immunoprecipitation).
8. Jung S, Kramer S, Schluesener HJ, Hunig T, Toyka K, Hartung HP. Prevention and therapy of experimental autoimmune neuritis by an antibody against T cell receptors-alpha/beta. J Immunol. 1992; 148(12):3768-3775. (Clone-specific: Depletion).
9. Kiely PD, Thiru S, Oliveira DB. Inflammatory polyarthritis induced by mercuric chloride in the Brown Norway rat. Lab Invest. 1995; 73(2):284-293. (Clone-specific: Depletion).
10. Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol. 1995; 25(2):328-332. (Clone-specific: (Co)-stimulation).
11. Pieters RH, Punt P, Bol M, van Dijken JM, Seinen W, Penninks AH. The thymus atrophy inducing organotin compound DBTC stimulates TcR alpha beta-CD3 signalling in immature rat thymocytes. Biochem Biophys Res Commun. 1995; 214(2):552-558. (Clone-specific: (Co)-stimulation, Depletion, Immunohistochemistry).
12. Wang M, Qu X, Stepkowski SM, Chou TC, Kahan BD. Beneficial effect of graft perfusion with anti-T cell receptor monoclonal antibodies on survival of small bowel allografts in rat recipients treated with brequinar alone or in combination with cyclosporine and sirolimus. Transplantation. 1996; 61(3):458-464. (Clone-specific: Depletion).
13. Yoshino S, Cleland LG, Mayrhofer G, Brown RR, Schwab JH. Prevention of chronic erosive streptococcal cell wall-induced arthritis in rats by treatment with a monoclonal antibody against the T cell antigen receptor alpha beta. J Immunol. 1991; 146(12):4187-4189. (Clone-specific: Depletion).
14. Yoshino S, Cleland LG. Depletion of alpha/beta T cells by a monoclonal antibody against the alpha/beta T cell receptor suppresses established adjuvant arthritis, but not established collagen-induced arthritis in rats. J Exp Med. 1992; 175(4):907-915. (Clone-specific: Depletion, Immunohistochemistry).
参考图片
αβ TCR expression in spleen and thymus. Lewis splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833, left panels) and purified R73 (bottom left panel) monoclonal antibodies, followed by FITC-conjugated anti-mouse IgG1 mAb A85-1 (Cat. No. 553443, left panels). Lewis thymocytes were stained with purified mAb R73 (bottom right panel), followed by FITC-conjugated anti-mouse IgG1 mAb A85-1 (right panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Flow cytometric analysis of αβ TCR expression in rat spleen and thymus. Lewis splenocytes were simultaneously stained with PE Mouse Anti-Rat CD3 (Cat. No. 554911, left panels) and Purified Mouse Anti-Rat αβ T-Cell Receptor (Cat. No. 554911; bottom left panel), followed by FITC Rat Anti-Mouse IgG1 (Cat. No. 553443, left panels). Lewis thymocytes were stained with Purified Mouse Anti-Rat αβ T-Cell Receptor (bottom right panel), followed by FITC Rat Anti-Mouse IgG1 (right panels). Fluorescent histograms and two-color contour plots were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
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