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PE Mouse Anti-Human IP-10-6D4-D6-G2_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:03:16 • 抗体 • 阅读 26 • 标签
产品信息
荧光素标记
PE
抗原名称
IP-10
宿主
Mouse IgG2a, κ
免疫原
Recombinant human IP-10 protein
简单描述
The monoclonal antibody 6D4/D6/G2 reacts with human CXC chemokine, interferon gamma inducible protein 10 (IP-10). IP-10 is inducible in monocytes, keratinocytes and endothelial cells by IFN-γ. The immunogen used to generate the monoclonal antibody 6D4/D6/G2 was recombinant human IP-10 protein.
商品描述
The monoclonal antibody 6D4/D6/G2 reacts with human CXC chemokine, interferon gamma inducible protein 10 (IP-10). IP-10 is inducible in monocytes, keratinocytes and endothelial cells by IFN-γ. The immunogen used to generate the monoclonal antibody 6D4/D6/G2 was recombinant human IP-10 protein.
同种型
Mouse IgG2a, κ
克隆号
6D4/D6/G2
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
反应种属
Human (QC Testing)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Luster AD, Ravetch JV. Biochemical characterization of a gamma interferon-inducible cytokine (IP-10). J Exp Med. 1987; 166(4):1084-1097. (Clone-specific). 2. Luster AD, Unkeless JC, Ravetch JV. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature. 1985; 315(6021):672-676. (Clone-specific). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block).

参考图片

Expression of IP-10 by stimulated human monocytes. Human PBMC were stimulated for 24 hours with Human Interferon-γ (1500 U/ml final concentration; Cat. No. 554617) in the presence of GolgiStop™ (2 µM final concentration; Cat. No. 554724). The PBMC were harvested, fixed, permeabilized, and stained with 0.05 µg of PE-mouse anti-human IP-10 antibody (PE-6D4/D6/G2, Cat. No. 555049) following BD Pharmingen staining protocol (see Figure, left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, binding by the PE-6D4/D6/G2 antibody was blocked by preincubation of the fixed/permeabilized cells with excess unlabeled 6D4/D6/G2 antibody (5 µg; middle panel) prior to staining with the PE-6D4/D6/G2 antibody. The level of nonspecific staining was assessed using the PE-mouse IgG2a isotype control (0.05 µg; PE-G155-178; Cat. No. 554648; right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and verified using the unlabeled antibody blocking control.

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