Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS (1.0 µg/ml final concentration; Sigma, Cat. No.L-8274) in the presence of BD GolgiStop™ (2 µM final concentration; Cat. No. 554724). PBMCs were then harvested, stained with 0.25 µg of PE Mouse Anti-Human CD14 ( Cat. No. 555398), fixed, permeabilized, and subsequently stained with either 0.125 µg of FITC-anti-human IL-8 (left panel), or 0.125 µg of FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555057; middle panel). The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls (right panel).

Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS (1.0 µg/ml final concentration; Sigma, Cat. No.L-8274) in the presence of BD GolgiStop™ (2 µM final concentration; Cat. No. 554724). PBMCs were then harvested, stained with 0.25 µg of PE Mouse Anti-Human CD14 ( Cat. No. 555398), fixed, permeabilized, and subsequently stained with either 0.125 µg of FITC-anti-human IL-8 (left panel), or 0.125 µg of FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555057; middle panel). The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls (right panel).