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BD Pharmingen- PE Mouse IgG2b- - Isotype Control_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:10:21 • 抗体 • 阅读 47 • 标签
产品信息
荧光素标记
PE
简单描述
This mouse IgG2b, κ isotype control is a monoclonal antibody, clone 27-35, that is specific for the dansyl (5-[dimethylamino] naphthalene-1-sulfonyl) hapten. The dansyl (DNS) hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing that demonstrated low background staining on a variety of mouse and human tissues.
商品描述
27-35 This mouse IgG2b, κ isotype control is a monoclonal antibody, clone 27-35, that is specific for the dansyl (5-[dimethylamino] naphthalene-1-sulfonyl) hapten. The dansyl (DNS) hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing that demonstrated low background staining on a variety of mouse and human tissues.
同种型
Mouse C.SW IgG2b, κ
克隆号
克隆 27-35 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
目标/特异性
IgG2b isotype control
背景
别名
Isotype Control (anti-Dansyl); Anti-Dansyl; Anti-DNS
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. BD Biosciences. Techniques for Immune Function Analysis, Application Handbook 1st Edition. 2003. Available: http://www.bdbiosciences.com/pdfs/manuals/02-8100055-21A1rr.pdf 2007, Jan. 25. 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology).

参考图片

Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (1.0 µg/ml final concentration) in the presence of GolgiStop™ (2 µM final concentration; Cat. No. 554724). The PBMC were harvested, stained with 0.25 µg of FITC-mouse anti-human CD14 monoclonal antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with either 0.125 µg of PE-anti-human IL-8 (Cat. No. 554720; left panel), or 0.125 µg of PE-mouse IgG2b (Cat. No. 555058; middle panel), following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls (right panel).

Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (1.0 µg/ml final concentration) in the presence of GolgiStop™ (2 µM final concentration; Cat. No. 554724). The PBMC were harvested, stained with 0.25 µg of FITC-mouse anti-human CD14 monoclonal antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with either 0.125 µg of PE-anti-human IL-8 (Cat. No. 554720; left panel), or 0.125 µg of PE-mouse IgG2b (Cat. No. 555058; middle panel), following BD PharMingen staining protocol. The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls (right panel).

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