This suspension contains H uman i ntracellular C yto K ine- 1 Positive Control Cells (HiCK-1). The HiCK-1 frozen cell suspension contains fixed , non-permeabilized human lymphoid cells. The suspension includes cells that express detectable levels of intracellular IL-2, IFN-γ and TNF as determined by immunofluorescent intracellular cytokine staining and flow cytometry. HiCK-1 cell suspensions were prepared by stimulating human PBMCs in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and fixed, then stored in 1ml of 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C.  HiCK-1 cells contain a measurable proportion of cytokines, with representative flow cytometric data shown below.  Performance from individual lots of HiCK-1 cells may differ due to donor variability. Investigators should anticipate similar, though not identical, results to those shown below.

This suspension contains H uman i ntracellular C yto K ine- 1 Positive Control Cells (HiCK-1). The HiCK-1 frozen cell suspension contains fixed , non-permeabilized human lymphoid cells. The suspension includes cells that express detectable levels of intracellular IL-2, IFN-γ and TNF as determined by immunofluorescent intracellular cytokine staining and flow cytometry. HiCK-1 cell suspensions were prepared by stimulating human PBMCs in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and fixed, then stored in 1ml of 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C.  HiCK-1 cells contain a measurable proportion of cytokines, with representative flow cytometric data shown below.  Performance from individual lots of HiCK-1 cells may differ due to donor variability. Investigators should anticipate similar, though not identical, results to those shown below.

(RUO)

5x10^6 cells/ml

Intracellular staining (flow cytometry) (Routinely Tested)

Hick-1 Cytokine positive control cells

Frozen in FBS and 10% DMSO.

研发参考(1) 1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block).