BD Pharmingen- HiCK-3 Human Cytokine Positive Control Cells_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:15:30 • 抗体 • 阅读 33 • 标签

产品信息

简单描述

This suspension contains H uman i ntracellular C yto K ine- 3 (HiCK-3) Positive Control Cells. The HiCK-3 frozen cell suspension contains fixed, non-permeabilized human lymphoid cells. The suspension includes cells that express detectable levels of intracellular IL-1α, IL-1β, IL-6, IL-12 p40/p70, IL-12 p70, and TNF as determined by immunofluorescent intracellular cytokine staining and flow cytometry. HiCK-3 cell suspensions were prepared by stimulating human PBMCs in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and fixed, then stored in 1 mL of 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C. HiCK-3 cells contain a measurable proportion of cytokines, with representative flow cytometric data shown below. Performance from individual lots of HiCK-3 cells may differ due to donor variability. Investigators should anticipate similar, though not identical, results to those shown below.

商品描述

This suspension contains H uman i ntracellular C yto K ine- 3 (HiCK-3) Positive Control Cells. The HiCK-3 frozen cell suspension contains fixed, non-permeabilized human lymphoid cells. The suspension includes cells that express detectable levels of intracellular IL-1α, IL-1β, IL-6, IL-12 p40/p70, IL-12 p70, and TNF as determined by immunofluorescent intracellular cytokine staining and flow cytometry. HiCK-3 cell suspensions were prepared by stimulating human PBMCs in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and fixed, then stored in 1 mL of 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C. HiCK-3 cells contain a measurable proportion of cytokines, with representative flow cytometric data shown below. Performance from individual lots of HiCK-3 cells may differ due to donor variability. Investigators should anticipate similar, though not identical, results to those shown below.

克隆号

(RUO)

浓度

5x10^6 cells/ml

应用

实验应用

Intracellular staining (flow cytometry) (Routinely Tested)

目标/特异性

Hick-3 Cytokine positive control cells

制备和贮存

存储溶液

Frozen in FBS and 10% DMSO.

保存方式

Frozen in FBS and 10% DMSO.

文献

文献

研发参考(2) 1. BD Biosciences. Techniques for Immune Function Analysis, Application Handbook 1st Edition. 2003. Available: http://www.bdbiosciences.com/pdfs/manuals/02-8100055-21A1rr.pdf 2007, Jan. 25. 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block).

参考图片

Flow cytometric staining of HiCK-3 Human Cytokine Positive Cells for IL-1α, IL-1β, IL-6, IL-12 and TNF. HiCK-3 cells were washed, permeabilized, and subsequently stained with either a PE-conjugated isotype control (upper far left panel), PE Mouse Anti-Human IL-1α (upper left middle panel, Cat. No. 554561), PE Mouse Anti-Human IL-1β (upper right middle panel, Cat. No. 340516), PE Rat Anti-Human IL-6 (upper far right panel, Cat. No. 554697), PE Mouse Anti-Human IL-12p40/p70 (lower far left panel, Cat. No. 554575), PE Rat Anti-Human IL-12 p70 (lower left middle panel, Cat. No. 557020), or PE Mouse Anti-Human TNF (lower right middle panel, Cat. No. 554513). Despite fixation and freezing, the side- and forward scattered light signals for these control cells (see far lower right panel) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown.

Flow cytometric staining of HiCK-3 Human Cytokine Positive Cells for IL-1α, IL-1β, IL-6, IL-12 and TNF. HiCK-3 cells were washed, permeabilized, and subsequently stained with either a PE-conjugated isotype control (upper far left panel), PE Mouse Anti-Human IL-1α (upper left middle panel, Cat. No. 554561), PE Mouse Anti-Human IL-1β (upper right middle panel, Cat. No. 340516), PE Rat Anti-Human IL-6 (upper far right panel, Cat. No. 554697), PE Mouse Anti-Human IL-12p40/p70 (lower far left panel, Cat. No. 554575), PE Rat Anti-Human IL-12 p70 (lower left middle panel, Cat. No. 557020), or PE Mouse Anti-Human TNF (lower right middle panel, Cat. No. 554513). Despite fixation and freezing, the side- and forward scattered light signals for these control cells (see far lower right panel) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown.

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