Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells.  Rat TNF is a 17.4 kD protein containing 157 amino acid residues.  Recombinant rat TNF (Cat. No. 555109) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives.  Recombinant rat TNF is ≥ 95% pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.  The endotoxin level is ≤ 0.1 ng per µg of TNF, as measured in a chromogenic LAL assay.

(RUO)

100 µg/ml

ELISA Standard (Routinely Tested), Bioassay (Tested During Development)

Rat (QC Testing)

TNF

Frozen aqueous buffered solution containing BSA.

Development References(2) 1. Hogan MM, Vogel SN. Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins. J Immunol. 1988; 141(12):4196-4202. (Methodology). 2. Jaattela, M. . Biologic activities and mechanisms of action of tumor necrosis factor-α/cachectin. Lab Invest. 1991; 64:724-742. (Biology).