产品信息
抗原名称
GM-CSF
克隆号
(RUO)
BD化合物表
- 描述数量/尺寸零件号EntrezGene ID
- Capture Antibody Purified Anti-Human GM-CSFN/A51-26091E1437
- Detection Antibody Biotin Anti-Human GM-CSFN/A51-26092E1437
- Recombinant Human GM-CSF Lyophilized StandardN/A51-26096E1437
- Enzyme Reagent Streptavidin-horseradish peroxidase conjugate (SAv-HRP)N/A51-9002813N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
GM-CSF
文献
文献
研发参考(9)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology).
3. Bacchetta R, de Waal Malefijt R, Yssel H. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990; 144(3):902-908. (Biology).
4. Gasson JC. Molecular physiology of granulocyte-macrophage colony-stimulating factor. Blood. 1991; 77(6):1131-1145. (Biology).
5. Kita H, Ohnishi T, Okubo Y, Weiler D, Abrams JS, Gleich GJ. Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils. J Exp Med. 1991; 174(3):745-748. (Biology).
6. Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Biology).
7. Lazzeri N, Belvisi MG, Patel HJ, Yacoub MH, Chung KF, Mitchell JA. Effects of prostaglandin E2 and cAMP elevating drugs on GM-CSF release by cultured human airway smooth muscle cells. Relevance to asthma therapy. Am J Respir Cell Mol Biol. 2001; 24(1):44-48. (Biology).
8. Miyatake S, Otsuka T, Yokota T, Lee F, Arai K. Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes. EMBO J. 1985; 4(10):2561-2568. (Biology).
9. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology).
参考图片
Representative standard curve using the BD OptEIA Human GM-CSF ELISA Set. A standard curve should be ran for each assay.
This standard curve is fordemonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.
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