产品信息
抗原名称
IL-10
克隆号
(RUO)
BD化合物表
- 描述数量/尺寸零件号EntrezGene ID
- Capture Antibody Purified Anti-Human IL-10N/A51-26171E3586
- Detection Antibody Biotin Anti-Human IL-10N/A51-26172E3586
- Recombinant Human IL-10 Lyophilized StandardN/A51-26176E3586
- Enzyme Reagent Streptavidin-horseradish peroxidase conjugate (SAv-HRP)N/A51-9002813N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
IL-10
文献
文献
研发参考(6)
1. Czerkinsky C, Andersson G, Ekre HP, Nilsson LA, Klareskog L, Ouchterlony O. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells. J Immunol Methods. 1988; 110(1):29-36. (Biology).
2. Czerkinsky CC, Nilsson LA, Nygren H, Ouchterlony O, Tarkowski A. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods. 1983; 65(1-2):109-121. (Biology).
3. Fujihashi K, McGhee JR, Beagley KW, et al. Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells. J Immunol Methods. 1993; 160(2):181-189. (Biology).
4. Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J Immunol. 2000; 164(7):3723-3732. (Biology).
5. Ronnblom L, Cederblad B, Sandberg K, Alm GV. Determination of herpes simplex virus-induced alpha interferon-secreting human blood leucocytes by a filter immuno-plaque assay. Scand J Immunol. 1988; 27(2):165-170. (Biology).
6. Sedgwick JD, Holt PG. A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods. 1983; 57(1-3):301-309. (Biology).
参考图片
This standard curve is for demonstration only. A standard curve must be run with each assay. Typical Standard Curve and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.
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