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BD OptEIA- Mouse IL-12 p40 ELISA Set_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:36:36 • 抗体 • 阅读 77 • 标签
产品信息
抗原名称
IL-12B (p40)
克隆号
(RUO)
BD化合物表
  • 描述
    数量/尺寸
    零件号
    EntrezGene ID
  • Capture Antibody Purified Anti-Mouse IL-12 P40/P70
    N/A
    51-26191E
    N/A
  • Detection Antibody Biotin Anti-Mouse IL-12 P40
    N/A
    51-26192E
    N/A
  • Recombinant Mouse IL-12 P40 Lyophilized Standard
    N/A
    51-26196E
    N/A
  • Enzyme Reagent Streptavidin-horseradish peroxidase conjugate (SAv-HRP)
    N/A
    51-9002813
    N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IL-12/IL-23 p40
文献
文献
研发参考(12) 1. Ashkar S, Weber GF, Panoutsakopoulou V, et al. Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. Science. 2000; 287(5454):860-864. (Biology). 2. Chougnet, et al. J Immunol. 2001; 3210-3217. (Biology). 3. D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G. Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells. J Exp Med. 1993; 178(3):1041-1048. (Biology). 4. D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Biology). 5. He J, Gurunathan S, Iwasaki A, Ash-Shaheed B, Kelsall BL. Primary role for Gi protein signaling in the regulation of interleukin 12 production and the induction of T helper cell type 1 responses . J Exp Med. 2000; 191(9):1605-1610. (Biology). 6. Iwasaki A, Kelsall BL. Unique functions of CD11b+, CD8 alpha+, and double-negative Peyer's patch dendritic cells. J Immunol. 2001; 166(8):4884-4890. (Biology). 7. Iwasaki A, et al. J Exp Med. 2000; 229-239. (Biology). 8. Ling P, Gately MK, Gubler U, et al. Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity. J Immunol. 1995; 154(1):116-127. (Biology). 9. Morelli AE, Larregina AT, Ganster RW, et al. Recombinant adenovirus induces maturation of dendritic cells via an NF-kappaB-dependent pathway. J Virol. 2000; 74(20):9617-9628. (Biology). 10. Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Biology). 11. Schoenhaut DS, Chua AO, Wolitzky AG, et al. Cloning and expression of murine IL-12. J Immunol. 1992; 148(11):3433-3440. (Biology). 12. Zeh HJ III, Tahara H, Lotze MT. Interleukin-12. In: Thomson AW, ed. The Cytokine Handbook. London: Academic Press Limited; 1994:239-256.

参考图片

This standard curve is for demonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (1000 pg/mL), then adding 100 µL of the 1000 pg/mL standard to both that well and the 500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 500 pg/mL standard to the 250 pg/mL well. Continue these dilutions to the 15.6 pg/mL standard well, out of which the extra 100 µL should be discarded.

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