This standard curve is for demonstration only. A standard curve must be run with each assay.\"Typical Standard Curve\" and 20-plate yield were obtained in thec BD Biosciences Pharmingen laboratory, using

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (2000 pg/mL), then adding 100 µL of the 2000 pg/mL standard to both that well and the 1000 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 1000 pg/mL standard to the 500 pg/mL well. Continue these dilutions to the 31.3 pg/mL standard well, out of which the extra 100 µL should be discarded.