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BD OptEIA- Mouse GM-CSF ELISA Set_BD Pharmingen

产品信息
抗原名称
GM-CSF
克隆号
(RUO)
BD化合物表
  • 描述
    数量/尺寸
    零件号
    EntrezGene ID
  • Recombinant Mouse GM-CSF Lyophilized Standard
    N/A
    51-26206E
    12981
  • Detection Antibody Biotin Anti-mouse GM-CSF
    N/A
    51-26202E
    12981
  • Capture Antibody Purified Anti-Mouse GM-CSF
    N/A
    51-26201E
    12981
  • Enzyme Reagent Streptavidin-horseradish peroxidase conjugate (SAv-HRP)
    N/A
    51-9002813
    N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
GM-CSF
文献
文献
研发参考(13) 1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology). 2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology). 3. Gasson JC. Molecular physiology of granulocyte-macrophage colony-stimulating factor. Blood. 1991; 77(6):1131-1145. (Biology). 4. Gough NM, Gough J, Metcalf D, et al. Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor. Nature. 1984; 309(5971):763-767. (Biology). 5. Miyatake S, Otsuka T, Yokota T, Lee F, Arai K. Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes. EMBO J. 1985; 4(10):2561-2568. (Biology). 6. Nabel G, Fresno M, Chessman A, Cantor H. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell. 1981; 23(1):19-28. (Biology). 7. Nabel G, Galli SJ, Dvorak AM, Dvorak HF, Cantor H. Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells. Nature. 1981; 291(5813):332-334. (Biology). 8. Nozaki S, Abrams JS, Pearce MK, Sauder DN. Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes. J Invest Dermatol. 1991 July; 97(1):10-14. (Biology). 9. Price V, Mochizuki D, March CJ, et al. Expression, purification and characterization of recombinant murine granulocyte-macrophage colony-stimulating factor and bovine interleukin-2 from yeast. Gene. 1987; 55:287-293. (Biology). 10. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 11. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Biology). 12. Suda T, O'Garra A, MacNeil I, Fischer M, Bond MW, Zlotnik A. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas. Cell Immunol. 1990; 129(1):228-240. (Biology). 13. Thompson-Snipes L, Dhar V, Bond MW, Mosmann TR, Moore KW, Rennick DM. Interleukin 10: a novel stimulatory factor for mast cells and their progenitors. J Exp Med. 1991; 173(2):507-510. (Biology).

参考图片

This standard curve is for demonstration only. A standard curve must be run with each assay. Typical Standard Curve and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100µL of Assay Diluent into each standard well except the highest (1000 pg/mL), then adding 100 µL of the 1000 pg/mL standard to both that well and the 500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 500 pg/mL standard to the 250 pg/mL well. Continue these dilutions to the 15.6 pg/mL standard well, out of which the extra 100 µL should be discarded.

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