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BD Pharmingen- Purified Rat Anti-Mouse CD3 Molecular Complex_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:58:07 • 抗体 • 阅读 54 • 标签
产品信息
抗原名称
CD3
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
免疫原
γδ TCR-positive T-T hybridoma D1
简单描述
The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
17A2 The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
克隆号
克隆 17A2 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Cytotoxicity, Immunohistochemistry-frozen, Immunoprecipitation, Stimulation (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
CD3
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3) 1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Immunogen: Cytotoxicity, Functional assay, Immunohistochemistry, Immunoprecipitation, Stimulation). 2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Clone-specific: Depletion). 3. Wu L, Antica M, Johnson GR, Scollay R, Shortman K. Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991; 174(6):1617-1627. (Biology: Cytotoxicity).

参考图片

CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with purified mAb 17A2 (bottom left panel) and PE-conjugated anti-mouse CD19 mAb 1D3 (top left & bottom left panels), followed by FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) (top left & bottom left panels). BALB/c thymocytes were also stained with 17A2 mAb (bottom right panel), followed by FITC-conjugated anti-rat IgG2b (top right & bottom right panels). Flow cytometry was performed on a BD FACSCan™ flow cytometry system.

CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with purified mAb 17A2 (bottom left panel) and PE-conjugated anti-mouse CD19 mAb 1D3 (top left & bottom left panels), followed by FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) (top left & bottom left panels). BALB/c thymocytes were also stained with 17A2 mAb (bottom right panel), followed by FITC-conjugated anti-rat IgG2b (top right & bottom right panels). Flow cytometry was performed on a BD FACSCan™ flow cytometry system.

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