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BD Pharmingen- FITC Rat Anti-Mouse CD3 Molecular Complex_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:58:58 • 抗体 • 阅读 119 • 标签
产品信息
荧光素标记
FITC
抗原名称
CD3
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
免疫原
γδ TCR-positive T-T hybridoma D1
简单描述
The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
商品描述
17A2 The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
克隆号
克隆 17A2 (RUO)
浓度
0.5 mg/ml
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD3
背景
别名
CD3; CD3 epsilon; Cd3e; CD3ε; T3e
制备和贮存
存储溶液
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Immunogen: Cytotoxicity, Functional assay, Immunohistochemistry, Immunoprecipitation, Stimulation). 2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Clone-specific: Depletion).
数据库链接
Entrez-Gene ID
12501

参考图片

CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with FITC Rat anti-Mouse CD3 Molecular Complex and PE Rat anti-Mouse CD19 (left panel). BALB/c thymocytes were also stained with FITC Rat anti-Mouse CD3 Molecular Complex (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with FITC-conjugated 17A2 (Cat. No. 555274) and PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399, left panel). BALB/c thymocytes were also stained with FITC-conjugated 17A2 mAb (Cat. No. 555274, right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

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