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BD Pharmingen- PE Rat Anti-Mouse CD3 Molecular Complex_BD Pharmingen

艾美捷科技 • 2025年08月22日 15:59:08 • 抗体 • 阅读 99 • 标签
产品信息
荧光素标记
PE
抗原名称
CD3
宿主
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
免疫原
γδ TCR-positive T-T hybridoma D1
简单描述
The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
商品描述
17A2 The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
同种型
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
克隆号
克隆 17A2 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD3
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2) 1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Biology: Induction). 2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Biology: Depletion).
数据库链接
Entrez-Gene ID
12501

参考图片

CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb 17A2 and FITC-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557398/553786, left panel). C3H/HeN thymocytes were stained with PE-conjugated 17A2 mAb (right panel, filled histogram) or unstained (right panel, empty histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb 17A2 and FITC-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557398/553786, left panel). C3H/HeN thymocytes were stained with PE-conjugated 17A2 mAb (right panel, filled histogram) or unstained (right panel, empty histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

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