1. 首页
  2. 抗体
  3. 正文

BD Pharmingen- Purified Mouse P-Selectin - IgG Fusion Protein_BD Pharmingen

艾美捷科技 • 2025年08月22日 16:06:16 • 抗体 • 阅读 79 • 标签
产品信息
宿主
Mouse IgG1
简单描述
The Mouse P-selectin - IgG Fusion Protein is composed of the signal sequence, the lectin domain, the EGF-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2 and CH3) of human IgG1. This chimeric protein binds to P-selectin ligand expressed on mouse neutrophils and myeloid cell lines. Furthermore, P-selectin-IgG fusion protein selectively stains Th1 cells, but not Th2 cells.reported in the literature.
商品描述
p-Selectin-Ig CHO The Mouse P-selectin - IgG Fusion Protein is composed of the signal sequence, the lectin domain, the EGF-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2 and CH3) of human IgG1. This chimeric protein binds to P-selectin ligand expressed on mouse neutrophils and myeloid cell lines. Furthermore, P-selectin-IgG fusion protein selectively stains Th1 cells, but not Th2 cells.reported in the literature.
同种型
IgG1
克隆号
克隆 p-Selectin-Ig CHO (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
CD62P (P-Selectin)
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(4) 1. Austrup F, Vestweber D, Borges E, et al. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues. Nature. 1997; 385(6611):81-83. (Biology). 2. Hahne M, Jager U, Isenmann S, Hallmann R, Vestweber D. Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes.. J Cell Biol. 1993; 121(3):655-664. (Biology). 3. Katakai T, Mori KJ, Masuda T, Shimizu A. Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis. Int Immunol. 2002; 14(2):167-175. (Biology). 4. Lenter M, Levinovitz A, Isenmann S, Vestweber D. Monospecific and common glycoprotein ligands for E- and P-selectin on myeloid cells. J Cell Biol. 1994; 125(2):471-481. (Biology).

参考图片

Detection of P-Selectin ligands on mouse granulocytes (left panel) and mononuclear cells (right panel). BALB/c whole blood was stained with Mouse P-Selectin - IgG Fusion Protein followed by R-PE-conjugated goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, open histogram) or with the second-step alone (filled histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555815/555816), and granulocytes and mononuclear cells were gated according to their light-scatter profiles. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Detection of P-Selectin ligands on mouse granulocytes (left panel) and mononuclear cells (right panel). BALB/c whole blood was stained with Mouse P-Selectin - IgG Fusion Protein followed by R-PE-conjugated goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, open histogram) or with the second-step alone (filled histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555815/555816), and granulocytes and mononuclear cells were gated according to their light-scatter profiles. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

文档下载: W 导出为BD Pharmingen- Purified Mouse P-Selectin - IgG Fusion Protein_BD Pharmingen.doc文档

本文来自投稿，不代表本人立场，如若转载，请注明出处：http://www.iamyjet.com/kangti/9849.html

赞 (79)
打赏 微信扫一扫
« 上一篇 2025年08月22日 16:04:57
下一篇 » 2025年08月22日 16:06:58

相关推荐

×
(function(){ var src = (document.location.protocol == "http:") ? "http://js.passport.qihucdn.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6":"https://jspassport.ssl.qhimg.com/11.0.1.js?1d7dde81dc0903e04d3ac0b9599444f6"; document.write('<\/mip-script>'); })(); (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();