BD Pharmingen- PE Mouse Anti-Human CD41a_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
CD41a
宿主
Mouse BALB/c IgG1, κ
免疫原
Purified platelet membrane glycoproteins
简单描述
The HIP8 monoclonal antibody specifically binds to the α-chain of CD41. CD41 is also known as Integrin αIIb or Platelet GPIIb. The calcium-dependent complex of CD41 and CD61 (β3 integrin or GPIIIa) is normally expressed on platelets and megakaryocytes. The CD41/CD61 complex is the receptor for fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation. CD41 (clone HIP8) completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.
Clone HIP8 also cross-reacts with a major subset of peripheral blood platelets of baboon, and both rhesus and cynomolgus macaque monkeys. The staining pattern of platelets is similar to that observed with peripheral blood platelets from normal human donors.
商品描述
HIP8
The HIP8 monoclonal antibody specifically binds to the α-chain of CD41. CD41 is also known as Integrin αIIb or Platelet GPIIb. The calcium-dependent complex of CD41 and CD61 (β3 integrin or GPIIIa) is normally expressed on platelets and megakaryocytes. The CD41/CD61 complex is the receptor for fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation. CD41 (clone HIP8) completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.
Clone HIP8 also cross-reacts with a major subset of peripheral blood platelets of baboon, and both rhesus and cynomolgus macaque monkeys. The staining pattern of platelets is similar to that observed with peripheral blood platelets from normal human donors.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 HIP8 (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)
目标/特异性
CD41a
背景
别名
ITGA2B; Integrin alpha-2b (αIIb); Platelet glycoprotein IIb (GPIIb)
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
参考图片
Flow cytometric analysis of CD41a expression on human peripheral blood platelets. Platelets were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human CD41a (Cat. No. 555467/557297/561424; solid line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™.
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