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BD Pharmingen- Biotin Mouse Anti-Human CD59_BD Pharmingen

产品信息
抗原名称
CD59
宿主
Mouse IgG2a, κ
免疫原
Human Erythrocytes
简单描述
The p282 (HI9) monoclonal antibody specifically binds to CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and Protectin. It inhibits the cytolytic activity of the complement system by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2, and plays a role in T-cell activation.
商品描述
p282 (H19) The p282 (HI9) monoclonal antibody specifically binds to CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and Protectin. It inhibits the cytolytic activity of the complement system by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2, and plays a role in T-cell activation.
同种型
Mouse IgG2a, κ
克隆号
克隆 p282 (H19) (RUO)
产品详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
CD59
背景
别名
HRF-20; MAC-inhibitory protein; MAC-IP; MACIF; MEM43; MIRL; Protectin; 1F5
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
966

参考图片

Flow cytometric analysis of glycosylphosphatidylinositol (GPI) anchor protein on GPI defective mutant cells. GPI defective mutant cells were stained with Biotin Mouse Anti-Human CD59 (Cat. No. 555762; solid line histogram) or Biotin Mouse IgG2a, κ Isotype Control (Cat. No. 555572; dashed line histogram), and PE Streptavidin (Cat. No. 554061). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™.

Flow cytometric analysis of glycosylphosphatidylinositol (GPI) anchor protein on GPI defective mutant cells. GPI defective mutant cells were stained with Biotin Mouse Anti-Human CD59 (Cat. No. 555762; solid line histogram) or Biotin Mouse IgG2a, κ Isotype Control (Cat. No. 555572; dashed line histogram), and PE Streptavidin (Cat. No. 554061). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™.

Flow cytometric analysis of glycosylphosphatidylinositol (GPI) anchor protein cells on K562 cells. K562 cells were stained with Biotin Mouse Anti-Human CD59 (Cat. No. 555762; solid line histogram) or Biotin Mouse IgG2a, κ Isotype Control (Cat. No. 555572; dashed line histogram) and PE Streptavidin (Cat. No. 554061). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™.

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