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BD Pharmingen- FITC Mouse Anti-Human CD59_BD Pharmingen

艾美捷科技 • 2025年08月26日 16:18:08 • 抗体 • 阅读 41 • 标签
产品信息
荧光素标记
FITC
抗原名称
CD59
宿主
Mouse IgG2a, κ
免疫原
Human Erythrocytes
简单描述
The p282 (HI9) monoclonal antibody specifically binds to CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and Protectin. It inhibits the cytolytic activity of the complement system by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2, and plays a role in T-cell activation.
商品描述
p282 (H19) The p282 (HI9) monoclonal antibody specifically binds to CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and Protectin. It inhibits the cytolytic activity of the complement system by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2, and plays a role in T-cell activation.
同种型
Mouse IgG2a, κ
克隆号
克隆 p282 (H19) (RUO)
产品详情
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
目标/特异性
CD59
背景
别名
HRF-20; MAC-inhibitory protein; MAC-IP; MACIF; MEM43; MIRL; Protectin; 1F5
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
文献
文献
研发参考(7) 1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997. 2. Davies A, Lachmann PJ. Membrane defence against complement lysis: the structure and biological properties of CD59. Immunol Res. 1993; 12(3):258-275. (Biology). 3. Deckert M, Kubar J, Bernard A. CD58 and CD59 molecules exhibit potentializing effects in T cell adhesion and activation. J Immunol. 1992; 148(3):672-677. (Biology). 4. Deckert M, Kubar J, Zoccola D, et al. CD59 molecule: a second ligand for CD2 in T cell adhesion. Eur J Immunol. 1992; 22(11):2943-2947. (Biology). 5. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997. 6. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995. 7. Whitlow MB, Iida K, Stefanova I, Bernard A, Nussenzweig V. H19, a surface membrane molecule involved in T-cell activation, inhibits channel formation by human complement. Cell Immunol. 1990; 126(1):176-184. (Biology).
数据库链接
Entrez-Gene ID
966

参考图片

Flow cytometric analysis of CD59 expression on human glycosylphosphatidiylinositol (GPI) anchor -defective cell line (left panel) or K562 cells expressing GPI anchor protein (right panel). Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histograms) or FITC Mouse Anti-Human CD59 antibody (Cat. No. 555763/560954; solid line histograms). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Fluorescent histograms depicting CD59 (or Ig isotype control) staining were derived from gated events with the forward and side-light scatter characteristics of K562 cells. Flow cytometric analysis was performed using a BD FACScan™ Cytometer System.

Flow cytometric analysis of CD59 expression on human glycosylphosphatidiylinositol (GPI) anchor -defective cell line (left panel) or K562 cells expressing  GPI anchor protein (right panel).  Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histograms) or FITC Mouse Anti-Human CD59 antibody (Cat. No. 555763/560954; solid line histograms). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Fluorescent histograms depicting CD59 (or Ig isotype control) staining were derived from gated events with the forward and side-light scatter characteristics of K562 cells. Flow cytometric analysis was performed using a BD FACScan™ Cytometer System.

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