BD Pharmingen- Purified Mouse Anti-Human RANTES_BD Pharmingen
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Immunocytochemical staining for RANTES. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37°C with LPS (Sigma Cat. No. L-8274, 1 µg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 550337) and the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011) (Nomarski optics, original magnification 400X).
Immunocytochemical staining for RANTES. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37°C with LPS (Sigma Cat. No. L-8274, 1 µg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 550337) and the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011) (Nomarski optics, original magnification 400X).
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