CD127

Rat IgG2a, κ

BALB/c mouse pre-B cell line 1A9

The SB/14 monoclonal antibody specifically binds to mouse CD127 which is also known as, the Interleukin-7 Receptor alpha chain (IL-7Rα). CD127 associates with CD132 (common γ chain) to form a high-affinity signaling IL-7R complex that mediates the biological effects of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR expression upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR+ T cells. The SB/14 mAb neutralizes IL-7-induced proliferation of the IL-7-dependent immature B-lymphocyte line 2E8 (detected by BrdU incorporation).

Rat IgG2a, κ

SB/14

1.0 mg/ml

Flow cytometry (Routinely Tested) Blocking (Reported) Immunoprecipitation, Western blot, Immunohistochemistry (Not Recommended)

Mouse (QC Testing)

Interleukin-7 receptor alpha chain; IL-7R alpha; IL7RA;IL-7RA; IL-7Rα; Il7r

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

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