BD Pharmingen- Purified Rat Anti-Mouse CD106_BD Pharmingen
产品信息
抗原名称
CD106
宿主
Rat LEW, also known as Lewis IgG2a, κ
免疫原
Mouse preadipose cell line PA6
简单描述
The 429 monoclonal antibody specifically binds to both the long (~110 kDa) transmembrane-spanning form and the truncated (~47 kDa) GPI-linked form of vascular cell adhesion molecule-1 (VCAM-1, CD106). CD106 is constitutively expressed on bone marrow stromal cells, myeloid cells, and splenic dendritic cells. Its expression on endothelial cells is upregulated by inflammatory cytokines and in certain pathologic conditions. CD106 expression has also been detected on apoptotic thymocytes, splenocytes, and lymphoid cell lines. VCAM-1 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin), and the 429 antibody partially blocks VCAM-1-mediated binding functions. Source of the immunogen was the mouse preadipose cell line PA6.
商品描述
429 (MVCAM.A)
The 429 monoclonal antibody specifically binds to both the long (~110 kDa) transmembrane-spanning form and the truncated (~47 kDa) GPI-linked form of vascular cell adhesion molecule-1 (VCAM-1, CD106). CD106 is constitutively expressed on bone marrow stromal cells, myeloid cells, and splenic dendritic cells. Its expression on endothelial cells is upregulated by inflammatory cytokines and in certain pathologic conditions. CD106 expression has also been detected on apoptotic thymocytes, splenocytes, and lymphoid cell lines. VCAM-1 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin), and the 429 antibody partially blocks VCAM-1-mediated binding functions. Source of the immunogen was the mouse preadipose cell line PA6.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 429 (MVCAM.A) (RUO)
浓度
31.25 µg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
反应种属
Mouse (QC Testing)
目标/特异性
CD106 (VCAM-1)
背景
别名
Vcam-1; Vascular cell adhesion molecule 1; Vascular cell adhesion protein 1
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology).
2. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994; 20(1):25-44. (Biology).
3. Kinashi T, St Pierre Y, Springer TA. Expression of glycophosphatidylinositol-anchored and -non-anchored isoforms of vascular cell adhesion molecule 1 in murine stromal and endothelial cells. J Leukoc Biol. 1995; 57(1):168-173. (Immunogen).
参考图片
Immunohistochemical staining of CD106. Frozen sections of normal mouse spleen were reacted with the anti-CD 106 antibody. Cells expressing CD106 can be identified by the brown labeling of their cell membranes. Amplification 20X.
Immunohistochemical staining of CD106. Frozen sections of normal mouse spleen were reacted with the anti-CD 106 antibody. Cells expressing CD106 can be identified by the brown labeling of their cell membranes. Amplification 20X.
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