BD Pharmingen- APC Mouse Anti-Human MIP-1- -CCL3_BD Pharmingen
产品信息
荧光素标记
APC
抗原名称
MIP-1α
宿主
Mouse IgG2a, κ
简单描述
The 11A3 monoclonal antibody specifically recognizes macrophage inflammatory protein-1α (MIP-1α), a member of the CC chemokine family of proteins. It is produced by T cells, B cells, Langerhans cells, neutrophils and macrophages. MIP-1α plays a role as an inhibitor of stem cell proliferation and as a chempattractant of B cells, eosinophils and cytotoxic T cells. Clone 11A3 also cross-reacts with an intracellular component of rhesus and cynomolgus macaque-LPS-stimulated peripheral blood monocytes. The reactivity pattern observed on CD14-positive cells is similar to that seen on normal human peripheral blood monocytes.
商品描述
11A3
The 11A3 monoclonal antibody specifically recognizes macrophage inflammatory protein-1α (MIP-1α), a member of the CC chemokine family of proteins. It is produced by T cells, B cells, Langerhans cells, neutrophils and macrophages. MIP-1α plays a role as an inhibitor of stem cell proliferation and as a chempattractant of B cells, eosinophils and cytotoxic T cells. Clone 11A3 also cross-reacts with an intracellular component of rhesus and cynomolgus macaque-LPS-stimulated peripheral blood monocytes. The reactivity pattern observed on CD14-positive cells is similar to that seen on normal human peripheral blood monocytes.
同种型
Mouse IgG2a, κ
克隆号
克隆 11A3 (RUO)
产品详情
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested)
推荐用量
20 µl
反应种属
Rhesus, Cynomolgus (QC Testing), Human (Tested in Development)
目标/特异性
MIP-1α
背景
别名
CCL3
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Rollins BJ. Chemokines. Blood. 1997; 90(3):909-928. (Biology).
2. Vaddi K, Keller M, Newton RC. The chemokine factsbook. San Diego: Academic Press; 1997:205 p.
3. Wolpe SD, Cerami A. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J. 1989; 3(14):2565-2573. (Biology).
参考图片
Flow cytometric analysis of MIP-1α (CCL3) expression on stimulated Rhesus macaque (Macaca mulatta) peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN-ɣ (10 ng/ml, 2 hours), then LPS (1 µg/ml, 2 hours) in the presence of GolgiStop™ (Cat. No. 554724; aka monensin 2 µM). The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1α (CCL3) (Cat. No. 551533). Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes.
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