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BD IMag- Anti-Mouse CD4 Magnetic Particles - DM_BD Pharmingen

艾美捷科技 • 2025年08月20日 11:00:03 • 抗体 • 阅读 27 • 标签
产品信息
抗原名称
CD4
宿主
Rat LEW, also known as Lewis IgG2b, κ
免疫原
Mouse CTL clone V4
简单描述
BD IMag™ anti-mouse CD4 Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD4-bearing leukocytes using the BD IMag™ Cell Separation Magnet. The CD4 (L3T4) differentiation antigen has been reported to be expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells.
商品描述
GK1.5 BD IMag™ anti-mouse CD4 Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD4-bearing leukocytes using the BD IMag™ Cell Separation Magnet. The CD4 (L3T4) differentiation antigen has been reported to be expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells.
同种型
Rat LEW, also known as Lewis IgG2b, κ
克隆号
克隆 GK1.5 (RUO)
应用
实验应用
Cell separation (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD4
背景
别名
Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(6) 1. Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). 2. Dialynas DP, Quan ZS, Wall KA, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983; 131(5):2445-2451. (Immunogen: Blocking, Depletion, Immunoprecipitation). 3. Dialynas DP, Wilde DB, Marrack P, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983; 74:29-56. (Clone-specific: Blocking, Depletion, Immunoprecipitation). 4. Frederickson GG, Basch RS. L3T4 antigen expression by hemopoietic precursor cells. J Exp Med. 1989; 169(4):1473-1478. (Biology). 5. Laronne-Bar-On A, Zipori D, Haran-Ghera N. Increased Regulatory versus Effector T Cell Development Is Associated with Thymus Atrophy in Mouse Models of Multiple Myeloma. J Immunol. 2008; 181(5):3714-3724. (Biology: Cell separation). 6. Wineman JP, Gilmore GL, Gritzmacher C, Torbett BE, Muller-Sieburg CE. CD4 is expressed on murine pluripotent hematopoietic stem cells. Blood. 1992; 180(7):1717-1724. (Biology).
数据库链接
Entrez-Gene ID
12504

参考图片

Positive selection and depletion of mouse CD4-positive splenocytes. Leukocytes were labeled with BD IMag™ anti-mouse CD4 Magnetic Particles - DM (Cat. No. 551539) as described in the protocol. After labeling, the cells were separated using the BD IMag™ Cell Separation Magnet (Cat. No. 552311), and the negative (CD4-) and positive (CD4+) fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh splenocytes (left panel), the negative fraction (middle panel), and the positive fraction (right panel) were stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553061) and PE Rat Anti-Mouse CD4 (Cat. No. 553048). The percent CD4+ cells in each sample is given in the upper right corner.

Positive selection and depletion of mouse CD4-positive splenocytes. Leukocytes were labeled with BD IMag™ anti-mouse CD4 Magnetic Particles - DM (Cat. No. 551539) as described in the protocol. After labeling, the cells were separated using the BD IMag™ Cell Separation Magnet (Cat. No. 552311), and the negative (CD4-) and positive (CD4+) fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh splenocytes (left panel), the negative fraction (middle panel), and the positive fraction (right panel) were stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553061) and PE Rat Anti-Mouse CD4 (Cat. No. 553048). The percent CD4+ cells in each sample is given in the upper right corner.

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