BD Pharmingen- PE Hamster Anti-Mouse TCR V-3_BD Pharmingen
产品信息
荧光素标记
PE
抗原名称
TCR Vβ3
宿主
Armenian Hamster IgG2, κ
免疫原
αβ TCR purified from mouse T-cell hybridoma 2B4.6
简单描述
The KJ25 antibody specifically reacts with the Vβ 3 T-cell Receptor (TCR) of strains having the
a
(e.g., C57BR, SJL),
b
(e.g., AKR, CBA/Ca, C57BL, DBA/1), and
c
(e.g., RIII) haplotypes of the
Tcrb
gene complex. Vβ 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the
Mtv-1
(
Mls-4[a], Mls[c]
),
Mtv-3
(
Mls[c]
),
Mtv-6
(
Mls-3[a], Mls[c]
),
Mtv-13
(
Mls-2[a], Mls[c]
),
Mtv-27
,
Mtv-44
, and/or
Mtv-MAI
endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). Vβ 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of Vβ 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.
商品描述
KJ25
The KJ25 antibody specifically reacts with the Vβ 3 T-cell Receptor (TCR) of strains having the
a
(e.g., C57BR, SJL),
b
(e.g., AKR, CBA/Ca, C57BL, DBA/1), and
c
(e.g., RIII) haplotypes of the
Tcrb
gene complex. Vβ 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the
Mtv-1
(
Mls-4[a], Mls[c]
),
Mtv-3
(
Mls[c]
),
Mtv-6
(
Mls-3[a], Mls[c]
),
Mtv-13
(
Mls-2[a], Mls[c]
),
Mtv-27
,
Mtv-44
, and/or
Mtv-MAI
endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). Vβ 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of Vβ 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.
同种型
Armenian Hamster IgG2, κ
克隆号
克隆 KJ25 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TCR Vβ3
背景
别名
TCR V beta 3; TCR Vβ3
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(8)
1. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology).
2. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology).
3. McCormack JE, Callahan JE, Kappler J, Marrack PC. Profound deletion of mature T cells in vivo by chronic exposure to exogenous superantigen. J Immunol. 1993; 150(9):3785-3792. (Biology).
4. Pullen AM, Marrack P, Kappler JW. The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens. Nature. 1988; 335(6193):796-801. (Immunogen).
5. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology).
6. Tomonari K, Fairchild S, Rosenwasser OA. Tcrb-V3+ T-cell deletion and a mouse mammary tumor provirus, Mtv-27. Immunogenetics. 1992; 36(5):302-305. (Biology).
7. White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. (Biology).
8. Yuuki H, Yoshikai Y, Kishihara K, et al. Deletion of self-reactive T cells in nude mice grafted with neonatal allogeneic thymus. J Immunol. 1990; 144(2):474-479. (Biology).
参考图片
Two-color analysis of the expression of Vβ 3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Hamster Anti-Mouse Vβ 3 T-Cell Receptor (Cat. No. 553209), FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047), and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Two-color analysis of the expression of TCR Vβ3 on mouse peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Hamster Anti-Mouse TCR Vβ3 (Cat. No. 553209), FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047), and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) monoclonal antibodies. The fluorescence contour plot was derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
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