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BD Pharmingen- Purified Rat Anti-Mouse IL-4_BD Pharmingen

艾美捷科技 • 2025年08月21日 16:27:06 • 抗体 • 阅读 17 • 标签
产品信息
抗原名称
IL-4
宿主
Rat IgG2b
免疫原
Recombinant Mouse IL-4
简单描述
The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This antibody is routinely tested by intracellular staining and sandwich ELISA. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
商品描述
BVD4-1D11 The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This antibody is routinely tested by intracellular staining and sandwich ELISA. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
同种型
Rat IgG2b
克隆号
克隆 BVD4-1D11 (RUO)
浓度
0.5 mg/ml
产品详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
应用
实验应用
ELISA Capture, Intracellular block/flow cytometry (Routinely Tested), Neutralization (Tested During Development), Western blot (Reported)
反应种属
Mouse (QC Testing)
目标/特异性
IL-4
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(5) 1. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization). 2. Finkelman FD, Madden KB, Morris SC, et al. Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes. J Immunol. 1993; 151(3):1235-1244. (Clone-specific: ELISA, Neutralization). 3. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Clone-specific: ELISA). 4. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Clone-specific: IC/FCM Block). 5. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA, Neutralization).

参考图片

Expression of IL-4 by MiCK-2 positive control cells. MiCK-2 positive control cells (Cat. No. 554653) were stained with 0.125 µg of FITC-conjugated rat anti-mouse IL-4 antibody by using the BD Pharmingen staining protocol (see left panel). To demonstrate specificity of staining, the binding of FITC-BVD4-1D11 was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-4 (0.25 µg, Cat. No. 550067; see middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled BVD4-1D11 antibody (5 µg, Cat. No. 554386; see right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

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